By Roberto Rodríguez-Díaz, Tim Wehr, Stephen F. Tuck
This reference assists scientists within the choice and alertness of analytical ideas for biopharmaceutical identity, formula, and assessment-providing a useful survey of exploratory equipment in most cases applied in the course of the preclinical, medical, and advertisement stages of improvement.
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27 Copyright © 2005 by Marcel Dekker. 28 Wehr Together, these characteristics of RPLC make it the preferred analytical technique for assessing protein purity and for elucidating protein structure. Reversed-phase high-performance liquid chromatography (HPLC) can also be used alone or in concert with other chromatographic techniques for purification of proteins. For the separation of synthetic polypeptides or peptides derived by proteolysis of proteins, RPLC has no equal. Reversed-phase peptide mapping is the technique of choice for confirming protein structure and identity, for determining the sites and nature of posttranslational modifications, and for characterizing protein modifications and degradation products.
The native folded state can exist in either the mobile phase (Fm) or the stationary phase (Fs), as can the unfolded state (Um, Us). The equilibrium constants (k) for interconversions of the four species are indicated. M. Lu, K. L. Karger, J. , 359: 19 . ) The molecular forces that are involved in maintaining the folded state of a protein are substantially the same as those involved in retention and elution. When the magnitude of the interactions involved in protein binding exceeds that of folding, the chromatographic process can induce conformation changes that expose hydrophobic groups and create new contact areas.
5). , isoleucine, phenylalanine, leucine, and tryptophan) were most strongly retained. The three chromatograms indicate the total analysis time increased in the order of isopropanol, acetonitrile, and methanol. This demonstrates that methanol is the weakest solvent, isopropanol is the strongest solvent, and acetonitrile is of intermediate eluting power. The best peak shapes and highest efficiencies were observed with acetonitrile, reflecting lower mobile-phase viscosity and better mass-transfer kinetics obtained with this modifer.
Analytical techniques for biopharmaceutical development by Roberto Rodríguez-Díaz, Tim Wehr, Stephen F. Tuck